engleski

Analysis of microsatellite loci in hop (Humulus lupulus L.)

Hop (Humulus lupulus L.) is a dioecious perennial plant native to the Northern Hemisphere cultivated for its use in the brewing industry. To investigate the genetic diversity present in wild accessions in comparison to cultivated hops, microsatellite marker variation was assessed at four loci in 124 accessions of wild and cultivated hops. A total of 63 alleles were identified with an average of 15.7 alleles per locus and an average PIC of 0.64 over four loci. The UPGMA tree based on Dice distance matrix could be divided into ten different clusters. All North American wild accessions clustered together separately from the rest of the accessions at high genetic dissimilarity values. The majority of wild accessions of European origin formed a separate cluster showing genetic differences from cultivated hops. Results of the AMOVA analyses based on Dice distance matrix indicate that the cultivated hops differ significantly from the wild ones, although most of the variability (88.95%) was found within groups. Molecular variances within groups of cultivated and wild hops were homogeneous, suggesting that a similar level of molecular variability can be found in both groups of accessions.

hop (Humulus lupulus L.); microsatellite markers; germplasm; wild hops; AMOVA

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