engleski

Mapping of quantitative trait loci controlling broomrape (Orobanche crenata Forsk.) resistance in pea (Pisum sativum L.)

Broomrape (Orobanche crenata Forsk.) is a root parasite that represents the major constraint for pea (Pisum sativum L.) cultivation in the Mediterranean area and in the Middle East. The efficacy of available control methods is minimal and the breeding for broomrape resistance is considered as the most promising strategy of control. Only moderate levels of incomplete resistance are available in pea germplasm. In order to identify and map the quantitative trait loci (QTLs) controlling the trait, 115 F2 plants derived from the cross between a susceptible and a resistant parent were analysed using isozymes, RAPD and STS markers. F2-derived F3 lines were studied for broomrape resistance under field conditions. The linkage map consisted of 21 linkage groups. The linkage groups included 120 markers and covered 1770 cM of the pea genome. Two QTLs for broomrape resistance (Ocp1 and Ocp2) explaining only a moderate portion of the observed variation (9.6 and 11.4 %, respectively) were detected.

pea (Pisum sp.); broomrape (Orobanche crenata L.); linkage map; QTL analysis; resistance genes

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