engleski

CD83 positive dendritic cells in the normal human first trimester decidua

PROBLEM: Key functions of dendritic cells (DCs) include uptake and processing of antigen and priming of naive T cells. Although an exclusive lineage-specific marker of human DCs is still lacking, CD83 has been proven to be a suitable and selective cell-surface marker for mature DCs. Human CD83 is a 45-kDa glycoprotein and member of the Ig superfamily. Functionally, immature DCs in the periphery are specialized for antigen capture by endocytosis or macropinocytosis, wheraes maturing DCs lose these capacities but, in paralel, significantely enhance their stimulatory properties for naive T cells and NK cells. The interaction between the fetus and its mother are made possible by the development of specialized regions, feto-maternal interface, where maternal immunocompetent cells (NK cells, gdT cells, macrophages, dendritic cells) and fetal trophoblast cells are brought into close association to interact with each other. Among cells of innate immunity, decidual DC could have an important part in antigen handling and establishing tolerance at the materno-fetal interface. The aim of this study was to analyse the presence, distribution, origin and function of mature CD83 positive DCs in normal human first trimester decidua. METHODS: Experiments were performed on freshly isolated and/or cultured decidual cells from normal human first trimester decidual tissue. CD1a and CD83 expression was detected by immunohistology. Phenotyping of CD83+ DCs were performed by flow cytometry analysis. RESULTS: Using immunohistology and flow cytometry we have identified CD83+ DCs in human first trimester decidual tissue. Very few CD1a positive cells were detected in the decidual basalis tissue sections. Frequency of CD83 molecule is 3x higher then for CD1a molecule (1.3%+/- 0.5 SEM versus 0.5 % +/- 0.2 SEM) in keeping with immunohistochemical findings. We have shown that frequency of CD83+ cells in the suspension of decidual cells is 3.2% +/-0.4 SEM (n=12) while there is only 1.3% +/-0.5 SEM (n=10) CD83+ cells in the suspension of decidual cells immediately after isolation. Human CD83+ DCs are of myeloid origin (CD11c+high CD123low) and have a phenotype of mature DCs. CONCLUSIONS: We have characterized CD83+ cells (on the basis of their surface phenotype and ontogeny) at the materno-fetal interface as myeloid DCs which express high amount of co-stimulatory molecules and MHC class II molecules. These investigations are supported by grant No. 0062029 from Ministry of Science and Technology, Republic of Croatia.

Dendritic cells; Decidua; Materno-fetal interface

nije evidentirano