engleski

The role of tumor necrosis factor alpha in the generation of acute phase response and bone loss in rats with talc granulomatosis

Trabecular bone loss is the part of acute-phase response (APR) in rats with subcutaneous granulomatous inflammation induced by talc.We investigated the possible involvement of inflammatory cytokine, tumor necrosis factor alpha (TNF-alpha) in the pathogenesis of bone loss and other aspects of APR.Intraperitoneal administration of specific neutralizing antibodies to TNF-alpha or of recombinant cytokine indicated that TNF-alpha was the primary mediator of bone changes, evidenced as slower bone elogation rate, bone marrow hyperplasia, and decreased trabecular bone volume and osteoblast number in tibial metaphysis.Moreover, direct intraosseal administration of anti-TNF-alpha antibody neutralized tha effect of inflammation on bone.On the other hand, the serum indices of the APR (decreased zinc and iron concentrations, ACTH and C-reactive protein concentration) were not greatly affected after the administration of anti-TNF- alpha antibody, except for the normalization of the hypercupremia and weight loss.The data presented in this report demonstrated direct involvement of TNF-alpha in the generation of bone alterations during the development of APR in rats with talc granulomatosis.

Inflammation-induced bone loss ; Interleukin-1 ; ACTH ; Osteoblasts

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