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FAST PURIFICATION OF PLASMID DNA BY USING SHORT MONOLITHIC COLUMNS (CROSBI ID 497151)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Branović, Karmen ; Forčić, Dubravko ; Ivančić, Jelena ; Štrancar, Aleš ; Barut, Miloš ; Košutić Gulija, Tanja ; Zgorelec, Renata ; Mažuran, Renata FAST PURIFICATION OF PLASMID DNA BY USING SHORT MONOLITHIC COLUMNS // First Summer School on Monoliths for Biochromatography, Bioconversion and Solid Phase Synthesis / Štrancar, Aleš (ur.). Ljubljana: BIA Separation, 2004. str. 60-60-x

Podaci o odgovornosti

Branović, Karmen ; Forčić, Dubravko ; Ivančić, Jelena ; Štrancar, Aleš ; Barut, Miloš ; Košutić Gulija, Tanja ; Zgorelec, Renata ; Mažuran, Renata

engleski

FAST PURIFICATION OF PLASMID DNA BY USING SHORT MONOLITHIC COLUMNS

Anion-exchange chromatography is one of the most important methods in downstream processing of plasmid DNA, both as a process and an analytical technique. Separation of plasmid DNA on traditional particle based anion-exchange supports is usually slow. Moreover, such supports have a low capacity for plasmid DNA due to the steric exclusion effects. In this work, the separation of plasmid DNA using short monolithic columns, Convective Interaction Media, will be presented. It will be demonstrated that plasmid DNA can be purified from bacterial cells using alkaline lysis followed by chromatography on a very short weak anion-exchange chromatographic columns - disks - with good purity and quality within a short time. Furthermore, the separation of plasmid DNA from cell RNA can be carried out without the need of adding RNA-se. Fast and efficient method for in-process control of the purified plasmid will be described.

plasmid DNA; monolith

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Podaci o prilogu

60-60-x.

2004.

objavljeno

Podaci o matičnoj publikaciji

First Summer School on Monoliths for Biochromatography, Bioconversion and Solid Phase Synthesis

Štrancar, Aleš

Ljubljana: BIA Separation

Podaci o skupu

First Summer School on Monoliths for Biochromatography, Bioconversion and Solid Phase Synthesis

poster

06.06.2004-09.06.2004

Portorož, Slovenija

Povezanost rada

Kliničke medicinske znanosti