engleski

First Isolation of Rickettsia conorii from Human Blood in Croatia

A "suicide" polymerase chain reaction (PCR), and a shell vial culture were done on samples of ethylendiamine tetra-acetic acid (EDTA) and citrate-anticoagulated blood samples. Indirect immunofluorescence was performed on sera collected from 17 patients clinically diagnosed with MSF during summer in three consecutive years, from 1998 to 2000. Results. The primers used in PCR amplified the expected part of the rickettsia genomic DNA and Rickettsia conorii grew from the shell vial-cultured blood of a single patient. In 13 patients, the diagnosis was confirmed serologically by paired sera, whereas in 4 patients the diagnosis remained presumptive, since no paired sera were available. Analyzing sequences of the ompA and citrate synthase gene, respectively, derived from the shell vial isolate, a 100% similarity with Rickettsia conorii, strain Seven (Malish), was found. Conclusion. This is the first isolation of Rickettsia conorii from a human sample in Croatia. Beside PCR-based methods and isolation, correct diagnosis of MSF could be still routinely reached by serology.

Boutonneuse fever; polymerase chain reaction; Rickettsia conorii; sequence analysis

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