Oxygenation alters ganglioside expression in rat liver following partial hepatectomy (CROSBI ID 106179)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Markotić, Anita ; Čikeš Čulić, Vedrana ; Tičinović Kurir, Tina ; Meisen, Iris ; Büntemeyer, Heino ; Boraska, Vesna ; Zemunik, Tatijana ; Petri, Nadan ; Peter-Katalinić, Jasna ; Mesarić, Marko ; Müthing, Johannes
engleski
Oxygenation alters ganglioside expression in rat liver following partial hepatectomy
Gangliosides from livers of weanling rats were analysed after 15% partial hepatectomy (PH) and different pre- and post-operative hyberbaric oxygenation (pre- and postHBO). Neu5Ac was the predominant ganglioside-derived sialic acid (>85%) compared to Neu5Gc. Almost identical low total sialic acid content (Neu5Ac+Neu5Gc) of the control and operated nonHBO animals opposed a 6.4 to 7.6 fold increase in pre- and postHBO animals (69.26 and 81.64 pmol per mg wet weight, respectively). NanoESI-QTOF mass spectrometry combined with HPTLC immunostaining revealed GM3(Neu5Ac) and GM3(Neu5Gc) as major gangliosides, correlating with the respective sialic acid concentrations. Minor neolacto- series gangliosides were enhanced in preHBO and postHBO, but GM1-core gangliosides only in preHBO rats. GM2 and GalNAc-GM1b were clearly detectable in oxygenated rats compared to traces in the control and nonHBO animals. These results point at a functional role of gangliosides in liver growth regulation and reconstitution after PH combined with pre- and post-operative HBO treatment.
HPLC ; HPTLC ; immunostaining ; nanoESI-QTOF mass spectrometry ; sialic acid ; partial hepatectomy ; oxygenation ; gangliosides
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Podaci o izdanju
330 (1)
2005.
131-141
objavljeno
0006-291X
10.1016/j.bbrc.2005.02.139