engleski

Effects of cortisole on inhibitory effect of halothane on human tumour cells, in vitro study

The aim of this in vitro assay is to evaluate whether addition of cortisole in dose observed intra-operatively modifies cell growth inhibition achieved by inhaled anaesthetic halothane. Human breast carcinoma (Zr-75, SKBr), human large bowel carcinoma (CaCo2) cells and normal fibroblasts (WI38) with cortisole 3μ g ml-1 were exposed to halothane 1.5 vol. in O2: N2O 35:60% and CO2 5% respectively. Exposition was performed 3 times at 37 C for 6 hours. Cytotoxicity of anaesthetics was analysed by using of validated tetrazolium dye assay (MTT): Cells were incubated at 37 C, after 24 hours 20 L of 5 mg/ml MTT in phosphate buffered saline was added to each well. Medium was removed after 4 hours and cells were washed out. The plates were transferred to Elisa reader (Stat fax 2100) to measure the dye extracted at 570 nm. Three groups of cells were analysed: exposed to halothane, to cortisole and halothane and incubated with cortisole alone. Differences of 20% and more were assumed as significant. Results observed in assay are shown on fig 1. There was not found a signifficant differece between cell lines exposed to halothane and cortisole, and halothane alone (correl = 0.9716). Addition of cortisole does not modify inhibitory effects of halothane to tumour cells growth. Reference Elena G, Puig NR. Bay ML. et al. Int J Immunopharmacol. 1997 ; 19:699-707.

cortisol; inhaled anaesthetics; antitumour effects

Rad je kap predavanje prezentiran i na skupu 10th European Society of Anaesthesiologists (ESA) Anniversary Meeting and 24th ESA Annual Meeting Euroanaesthesia 2002, održanom od 06.09.2002., Nica, Francuska ; sažetak je objavljen u European Journal of Anaesthesiology. Supplement 19 (2002) (S24) / A.P. Adams (ur.). ; 2002. 114-114.