engleski

Influence of transduced p27 on cell cycle

Plasma membranes of cells are generally impermeable to proteins and peptides. The potential for intracellular therapeutic use of proteins, peptides and oligonucleotides has been limited by the impermeable nature of the cell membrane to these compounds. To achieve an efficient intracellular drug and DNA delivery, attempts were made to target microparticulate drug carriers into cytoplasm bypassing the endocytotic pathway. TAT peptides derived from the HIV-1 TAT protein facilitate intracellular delivery of proteins and small colloidal particles. TAT protein enters into the cells when added to the surrounding media. Protein transduction has been widely used to analyze biochemical processes in living cells. The present study analyzed the effects of cell cycle on the uptake of proteins responsible for regulation of cell cycle. The proteins (p27, p23, Mp27) were transdused into different cell lines (NALM, MOLT, Raji, SuDHL, and K562) and their effects on proliferation of the cells were measured. A transduced p27 did not remarkable influence on proliferation of examined cell lines. Mutated p27 inhibited the proliferation of examined cell lines up to 30 %. On the other hand, a transduction of p23 protein, truncated form of p27, inhibited the proliferation all of examined cell lines 30-60 %. Also the effects on expression of host p27 protein were examined, as well as an influence on induction of apoptosis.

cell cycle; p27

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