Physiologic and pathogenetic significance of perforin in pregnancy (CROSBI ID 467053)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Rukavina, Daniel ; Laškarin, Gordana ; Štrbo, Nataša ; Sotošek, Vlatka ; Podack, Eckhard R.
engleski
Physiologic and pathogenetic significance of perforin in pregnancy
Perforin (P) positive lymphocytes are present in high concentration in the metrial gland of rodents' pregnancy and first trimester human pregnancy decidua. Experiments with P deficient mice showed that P plays a major role in CTL and NK cell-mediated cytotoxicity, but is not necessary for normal pregnancy. However, mice simultaneously deficient for both cytolytic pathways (Fas L/Fas and Perforin double deficient mice-DD) are short lived and female mice are infertile. Mice homozygous for Fas-L defect, but heterozygous at the P locus and expressing one functional P allele are fertile and protected from early death. The analyses of P expression in peripheral blood lymphocytes (PBL) of nonpregnant (NP) first trimester pregnancy (FTP) and term pregnancy (TP) females showed considerable differences: 1) Proportion of P+ cells increased significantly at the end of the pregnancy compared to FTP (22% vs. 37%) ; 2) In the FTP 18% of P+ cells are CD4+ which is 3 times higher than in NP ; 3) drastic decrease of CD16+ cells in the population of P+ cells (from 45% in NP to 4% to FTP), and increase at TP (67%). In the FTP decidua CD3 CD56 bright+CD16    decidual lymphocytes (DL) are Pbright+, which was confirmed by analyzing CD3 clones. In pathological pregnancies (anembryonic pregnancy and missed abortion) both the percentage of CD4+ cells among P+ cells and the content of P/cell were 3 times lower compared to NP. Decidual adherent cells (DAC) and/or their supernatants have pivotal role in upregulation of P expression in decidual lymphocytes. These effects can be blocked by anti IL-15 antibodies. In our opinion direct and indirect (cytokine and hormone mediated) interactions between DAC and decidual lymphocytes is responsible for high level of P expression in DL.
perforin; pregnancy; physiology; pathophisology
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Podaci o prilogu
250-250.
1998.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
Abstracts of the VII International Congress of Reproductive Immunology ; u: American Journal of Reproductive Immunology
Gleicher, Norbert
Kopenhagen: Munksgaard
Podaci o skupu
International Congress of Reproductive Immunology (7 ; 1998)
pozvano predavanje
27.10.1998-30.10.1998
New Delhi, Indija