engleski

DRB1*01 in the presence of MICA-A9 ALLELE is associated to IDDM in croatians negative for DRB1*03 AND *04 ALLELES

Polymorphism in the exon 5 of the MHC class I chain related gene-A (MICA) has been reported to be a new genetic marker in the predisposition to insulin dependent diabetes mellitus (IDDM). In this study we analysed MICA polymorphism in 94 patients with IDDM and 184 healthy, unrelated controls. HLA typing for class II genes was performed with PCR-SSP method. MICA polymorphism was analysed using electrophoresis of the PCR amplified samples on a 6% polyacrylamide gel. Distribution of MICA alleles demonstrated no statistically significant difference between the entire groups of controls and patients. For the further study, we divided patients and controls into two groups on the basis of their DRB1 typing: DRB1*03 and/or *04 positive and DRB1*03 and *04 negative individuals. The analysis revealed that among patients negative for DRB1*03 and *04, DRB1*01 and MICA-A9 were present with a higher frequency when compared to the matched controls (p=0.008, and p=0.03, respectively), as well as when compared to the patients positive for DRB1*03 and/or *04 (p=0.003, p<0.001, respectively). The presence of both, DRB1*01 and MICA-A9, was found in 40% of DRB1*03 and *04 negative patients which was significantly higher than in matched controls (p=0, 003). These results suggest that DRB1*01 in the presence of MICA-A9 might be considered as a predisposing genetic factor to IDDM in DRB1*03 and DRB1*04 negative individuals.

HLA; MICA-A9 ALLELE; IDDM; CROATIANS

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