engleski

Changes in T-cell subpopulations during 4 years of suppression of HIV-1 replication in patients with advanced disease

Background: The aim of our study was to compare the number/% of naive and memory CD4+ T-cells, CD38+CD8+ T-cells and CD28+CD4+ and CD28+CD8+ T-cells in patients with advanced (baseline CD4+ count < 100 cells) with those with less advanced (baseline CD4+ cell count > 100 cells) HIV-disease during 4 years of suppressive (<200 copies/ml) HAART. Methods: This prospective longitudinal study included 24 treatment-naive patients and 32 controls. Cells were analysed by flow cytometry at baseline and after 1, 3, 6, 12, 24 and 48 months (mo) of HAART. The within-group and between group comparisons were made by exact nonparametric tests. Results: Advanced HIV-infected patients (n=13 ; median baseline CD4+: 23/&micro ; ; l) gained more CD4+ T-cells than less advanced patients (n=11 ; median baseline CD4+:189/&micro ; ; l) at 1 mo (median: 60 vs 36/&micro ; ; l), 3 mo (86 vs 14), 6 mo (111 vs 23), 12 mo (174 vs 47), 24 mo (162 vs 72) and 48 mo (257 vs 123), (p=0.15 ; p<0.001 ; p=0.026 ; p=0.021 ; p=0.1 ; p=0.06 respectively). Advanced patients gained relatively more naive CD4+ T-cells at 48 mo compared to less advanced patients (27.3 vs 11.4%, p=0.05). The relative gain in memory CD4+ T-cells was greater in advanced versus less advanced patients at 1 mo (median: 6.4 vs 1.4%), 3 mo (4.3 vs 2.0), 6 mo (6.7 vs 1.6), 12 mo (6.9 vs 2.4), 24 mo (7.5 vs 3.1) and 48 mo (11.3 vs 6.8) (p=0.002 ; p=0.013 ; p<0.001 ; p=0.004 ; p=0.001 ; p=0.015, respectively). There was no difference in CD38+CD8+ T-cells, CD28+CD4+ and CD28+CD8+ T-cells between advanced and less advanced patients. At 48 mo, CD38+CD8+ T-cells reached normal values in both advanced (4.7%, p=0.309 vs controls) and less advanced patients (9.2%, p=0, 869 vs controls). Naive CD4+ T-cells in less advanced patients (median 50.6%) were similar to controls (47.5% ; p=0.699) at mo 48. Conclusion: The kinetics of truly naive and memory CD4+ T-cell reconstitution during 4 years of suppressive HAART is different in less advanced compared to advanced HIV-patients.

T-cell; HIV-1

nije evidentirano