engleski

Combined early treatment with DMSA and DTPA to mobilize cadmium in rats

The influence of chelating agents: meso-2, 3-dimercaptosuccinic acid (DMSA) ; calcium trisodium diethylenetriaminepentaacetate (DTPA) and their combination on tissue retention and distribution of cadmium (Cd) was compared in female albino rats. Special attention was given to time of chelators application after cadmium administration. After oral cadmium intubation chelators were applied either orally (DMSA) or intraperitoneally (DTPA) in various short time intervals after cadmium. The dose of cadmium chloride was 0, 25 mmol/kg body weight and chelators dose was 1 mmol/kg, each. Three experiments were carried out with four treatment groups in each of them: 1) Cd (control) ; 2) Cd + DMSA ; 3) Cd + DTPA ; 4) Cd + DMSA + DTPA. Time intervals for chelator application after cadmium administration were: immediately in the first, half an hour in the second and one hour in the third experiment. Cadmium, iron, copper and zinc were measured in 24-hour urine collected after chelators application and in organs (liver, kidney and brain) at the end of each experiment. Results showed that the efficiency of cadmium removal from the body is lower when the time of chelator application is longer after cadmium administration. The two chelators differ in efficiency in mobilizing cadmium, with DMSA being more efficient than DTPA. The combined therapies of two chelators give slightly better results of cadmium chelation. It seems that DMSA that is given orally after oral cadmium removes this element very efficiently from the gastrointestinal tract. However, DTPA which is given parenterally removes absorbed cadmium very modestly. Whenever DTPA was given to animals zinc concentration is significantly higher in kidneys and much higher in urine than in other groups. Iron and copper do not change dramatically after chelation treatment.

chelators; DMSA; DTPA; cadmium; rat

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