engleski

DISTRIBUTION OF ALLELES AT THREE MICROSATELLITE LOCI (D6S265, D6S273, AND MIB) AMONG PATIENTS SUFFERING FROM TYPE I DIABETES

Type l diabetes is an autoimmune disease caused by destruction of the insulin producing  cells in pancreas. Both genetic and environmental factors participate in the development of disease. Recent studies have indicated the existence of non-HLA loci associated with type I diabetes in addition to previously known association with the HLA class II genes. One hundred patients selected on the basis of their HLA alleles, where classified in 4 groups (DR3/DR4, DR3, DR4, non DR3/DR4). Control group consisted of 150 healthy individuals. PCR-SSP method was performed for HLA class II typing, while 3 microsatellite loci (D6S265, D6S273, and MIB}; were analysed using automated ALFexpress sequencer. Analysis of the allele distribution revealed differences between patients and controls for D6S273 and MIB loci while there was no difference observed for D6S265 locus. Alleles D6S273-128bp (p=0.02565};, D6S273-138bp (p=0.01697), D6S273-140bp (p=0.00035), MIB-348bp (p=0, 01302), MIB350bp (p=0, 00677}; and MIB-352bp (p=0, 02289) were present with an increased frequency among patients, while alleles D6S273-130bp (p=0, 02945) and MIB-334bp (p=0, 00105) appeared with decreased frequency. We also examined the possibility that these alleles showed association with disease as result of their linkage with HLA class II genes, by excluding individuals positive for DR3 and/or DR4 antigens from further analysis. Difference was then observed only for alleles D6S273-132bp (p=0, 03986};, MIB332bp (p=0, 04376}; and MIB-350bp (p=0, 01212};. Comparison of DR3 and DR4 positive groups showed that other alleles were in linkage disequilibrium with HLA class II genes. In conclusion, our results suggest two new possible susceptibility markers for type I diabetes: D6S273 and MIB loci.

IDDM; STR; PCR; HLA

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