engleski

Detection of Escherichia coli 0157 in food samples by Immunomagnetic separation in Zagreb area

Hemorrhagic Escherichia coli 0157 strains can pose a serious health threat in tained food.Traditional culture-baised methods for assay of this enteric pathogen in food is relatively slow and results can be ambiguous.A new detection system, the immunomagnetic separation has been developed to detect Escherichia coli 0157 in food.This method separates Escherichia coli 0157 cells from enriched food samples by means of immunomagnetic beads.After magnetic capture the beads are spread on SMAC agar.The analysis time, including an 6-h enrichment and plate incubation is 48 h.Between 1999. and 2002. in Zagreb area, 300 food samples were investigated for the presence of Escherichia coli 0157.The foodstuffs included 300 uncooked foods (100 raw vegetables, 100 raw cow's milk and 100 raw meats and meat-products.Escherichia coli 0157 was present only in one sample, VT1+.

Hemorrhagic strains; Escherichia coli; Immunomagnetic separation

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