engleski

Laboratory biotransformation studies of polychlorinated biphenyls for remediation of contaminated soil

The main objective of our study was to propose remediation method for enhancing PCB transformation in soil contaminated by the power substation oil spills. Biodegradation experiments were performed in microcosms by using enriched mixed and pure cultures originating from polluted area of the power substation, Zadar, Croatia. The enriched mixed culture (TSZ7) as well as its isolated member (strain Z6) showed substantial PCB degradation activity in mineral medium with the addition of biphenyl as carbon and energy source and the inducer of PCB degradation pathway (56.5-73.5% reduction of PCB congeners in commercial mixture containing two- to octachlorobiphenyls). Further PCB degradation experiments were performed in the presence of the following substrates: a) xylose as the carbon and energy source ; b) terpenes (carvone and limonene) as potential inducers of the dioxygenase metabolic pathway and c) saya lecithin, phytogenic surfactant for enhancing PCB bioavailability. The addition of those substrates exerted an enhancing effect on PCB-degradation activity of tested cultures under the laboratory conditions, which is promising and provide the basis for planning small-scale-field degradation experiments.

polychlorinated biphenyls; biodegradation; remediation

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