engleski

Avian thymic hormone treatment of peripheral mononuclear cells from young chicks stimulates acute graft-versus-host reaction in chicken embryos

Avian thymic hormone (ATH) is a parvalbumin produced by epithelial cells in the thymic cortex of chickens and circulates in the blood on a five-day cycle. It stimulates precocious development of cell-mediated immunity. The effect of partially purified extracts of thymus (TE) and purified ATH were tested for their effect on the acute graft-versus-host reaction (GVHR). Treatment of chicks for their first three days of life did not enhance the acute GVHR produced by their PBMC in 14-day-old embryos. PBMC from 3-day-old chicks were treated in vitro with TE, ATH, thymosin fraction 5 or thymosin alpha 1 for 2 h and injected into 14-day-old embryos. Bone marrow cells and thymic lymphocytes were treated with TE. Only PBMC treated with TE or ATH produced an enhanced acute GVHR. Because ATH targets gamma delta T cells, the data implicate participation of donor gamma delta T cells in the acute GVHR.

avian thymic hormone; peripheral blood mononuclear cells; graft-versus-host reaction; T cells; chicken

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