Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

Expression of bone morphogenetic proteins in stromal cells from human bone marrow long-term culture (CROSBI ID 109134)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Martinović, Snježana ; Mazić, Sanja ; Kisić, Veronika ; Bašić, Nina ; Jakić-Razumović, Jasminka ; Borovečki, Fran ; Batinić, Drago ; Šimić, Petra ; Grgurević, Lovorka ; Labar, Boris et al. Expression of bone morphogenetic proteins in stromal cells from human bone marrow long-term culture // The journal of histochemistry and cytochemistry, 52 (2004), 9; 1159-1167-x

Podaci o odgovornosti

Martinović, Snježana ; Mazić, Sanja ; Kisić, Veronika ; Bašić, Nina ; Jakić-Razumović, Jasminka ; Borovečki, Fran ; Batinić, Drago ; Šimić, Petra ; Grgurević, Lovorka ; Labar, Boris ; Vukičević, Slobodan

engleski

Expression of bone morphogenetic proteins in stromal cells from human bone marrow long-term culture

Highly purified primitive hemopoietic stem cells express BMP receptors but do not synthesize bone morphogenetic proteins (BMPs). However, exogenously added BMPs regulate their proliferation, differentiation, and survival. To further explore the mechanism by which BMPs might be involved in hemopoietic differentiation, we tested whether stromal cells from long-term culture (LTC) of normal human bone marrow produce BMPs, BMP receptors, and SMAD signaling molecules. Stromal cells were immunohistochemically characterized by the presence of lyzozyme, CD 31, factor VIII, CD 68, S100, alkaline phosphatase, and vimentin. Gene expression was analyzed by RT-PCR and the presence of BMP protein was confirmed by immunohistochemistry (IHC). The supportive role of the stromal cell layer in hemopoiesis in vitro was confirmed by a colony assay of clonogenic progenitors. Bone marrow stromal cells express mRNA and protein for BMP-3, -4, and -7 but not for BMP-2, -5, and -6 from the first to the eighth week of culture. Furthermore, stromal cells express the BMP type I receptors, activin-like kinase-3 (ALK-3), ALK-6, and the downstream transducers SMAD-1, -4, and -5. Thus, human bone marrow stromal cells synthesize BMPs, which might exert their effects on hemopoietic stem cells in a paracrine manner through specific BMP receptors.

bone marrow; long-term culture (LTC); BMP

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

52 (9)

2004.

1159-1167-x

objavljeno

0022-1554

Povezanost rada

Temeljne medicinske znanosti, Kliničke medicinske znanosti

Indeksiranost