engleski

Micronuclei in cytokinesis-blocked lymphocytes as an index of occupational exposure to antineoplastic drugs

In order to investigate possible DNA damaging effects of antineoplastic drugs, the micronucleus assay combined with Giemsa, DAPI and silver staining was performed. Blood samples were taken from nurses working without adequate protection in oncological department on preparing and administration of different antineoplastic drugs. Lymphocytes were cultivated in vitro at 37oC. To prevent cytokinesis at 44h cytochalasine-B in final concentration of 3 g/ml was added. The results obtained indicate statistically significant increases in total number of micronuclei in exposed subjects compared to control. DAPI staining has revealed signal-positive and signal-negative micronuclei while silver staining has revealed Ag-NOR+ and Ag-NOR- micronuclei. Compared to control the number of signal-positive and Ag-NOR+ micronuclei in exposed subjects were increased indicating greater susceptibility of particular chromosomes to damage caused by antineoplastic agents.

antineoplastic agents-adverse effects; occupational exposure; lymphocytes; micronucleus test

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