Genetic characterization of L-Zagreb mumps vaccine strain (CROSBI ID 503209)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Ivančić, Jelena ; Košutić Gulija, Tanja ; Forčić, Dubravko ; Baričević, Marijana ; Jug, Renata ; Meško-Prejac, Majda ; Mažuran, Renata
engleski
Genetic characterization of L-Zagreb mumps vaccine strain
Eleven mumps vaccine strains, all containing live attenuated virus, have been used throughout the world. Although L-Zagreb mumps vaccine has been licensed since 1972, only its partial nucleotide sequence was previously determined (Acc. Nos. AJ272363, AY623666 and AY583323). Therefore, we sequenced the entire genome of L-Zagreb vaccine strain (Institute of Immunology Inc., Zagreb, Croatia). In order to investigate the genetic stability of the vaccine, sequences of both L-Zagreb master seed and currently produced vaccine batch were determined and no difference between them was observed. A phylogenetic analysis based on SH gene sequence has shown that L-Zagreb strain does not belong to any of established mumps genotypes and that it is most similar to old, laboratory preserved European strains (1950'-1970'). L-Zagreb nucleotide and deduced protein sequences were compared with other mumps virus sequences obtained from the GenBank. Emphasis was put on functionally important protein regions and known antigenic epitopes. The extensive comparisons of nucleotide and deduced protein sequences between L-Zagreb vaccine strain and other previously determined mumps virus sequences have shown that while the functional regions of HN, V, and L proteins are well conserved among various mumps strains, there can be a substantial amino acid difference in antigenic epitopes of all proteins and in functional regions of F protein. No molecular pattern was identified that can be used as a distinction marker between virulent and attenuated strains.
mumps; vaccine; genotype
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Podaci o prilogu
204-204-x.
2004.
objavljeno
Podaci o matičnoj publikaciji
Second European Congress of Virology, Eurovirology 2004
Esteban, Mariano ; Fernandez-Munoz, Rafael ; Carman, William
Madrid:
Podaci o skupu
Second European Congress of Virology, Eurovirology 2004
poster
05.09.2004-09.09.2004
Madrid, Španjolska