engleski

Investigation of the Antimutagenic effect of Cruciferae vegetables on bacterial test systems and human cell lines

Glucosinolates are a group of compounds widely distributed among the plants of the Cruciferae family. These compounds are easily hydrolysed by the enzyme mirosinase which becomes activated after mechanical destruction of the plant cells. Isothhiocyanates, nitriles, thiocyanates and indole compounds are the most common products of such hydrolysis. In our previous report we have shown that there was no cytrotoxic and mutagenic effect of indole-3-carbinol and cauliflower extract on two bacterial test systems, Salmonella typhimurium, strains TA987 and TA100. In this work we have examined potential inhibitory effect of both compounds against two standard mutagens , 2-aminoanthracene and 4-nitroquinoline-1-oxide. It was estimated that simultaneous treatment of bacterial cells with mutagen and indole compounds leads to a significant inhibition of prototrofic growth. Indole compounds didn’ t inhibit the activity of P-glycoprotein in parental laryngeal carcinoma cells (HEp2) and their cisplatin resistant subline (CK2). Indole compounds caused significant increase of glutathione level and gutathione-S-transferase activity in HEp2 cells. On the contrary, in CK2 cells which have increased basal level of glutathione, both drugs decreased the level of glutathione and glutathione-S-transferase activity. Indole-3-carbinol and cauliflower extract didn’ t cause any abberant event in human lymphocytes.

Cruiferae family; salmonella typhimurium; Indole-3-carbinol

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