Human papillomaviruses DNA infection in head and neck squamous cell carcinomas detected by dot-blot hybridization (CROSBI ID 81695)
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Cerovac, Željka ; Lučin, Ana ; Šarčević, Božena ; Kralj, Zvonimir ; Ban, Jasna
engleski
Human papillomaviruses DNA infection in head and neck squamous cell carcinomas detected by dot-blot hybridization
Human papillomaviruses (HPV) infection could be associated with the development of some human tumors of squamous epithelium. Twenty one squamous cell carcinomas (8 larynx, 3 tongue, 6 oral cavity and 4 pharynx), with different grading, were analyzed for the presence of HPV DNA by dot-blot hybridization under the stringent conditions with digoxigenin labelled probes for HPV types 6, 16 and 18. Positive HPV signals were found in DNA isolated from 10 invasive carcinomas (47.6%), 4 (19%) epithelia adjacent to carcinomas, and 2 (11.7%) resected margins. HPV-positive signals were obtained in 44% of oral cavity and tongue, in 62.5% of laryngeal, and in 25% of pharyngeal carcinomas. Out of 10 HPV- positive tumors a single HPV type was determined in 80%, and double infection in 20% cases. As the only type HPV 6 DNA was determined in two (20%), HPV 16 DNA in 5 (50%), and HPV 18 DNA in 1 (10%) head and neck carcinoma. The detection rate of HPV 6 was lower than of HPV 16 or 18 for all tumor sites. Among all tumors HPV DNA was determined in 57% histologically graded as well differentiated, 20% as moderately differentiated, and 55% as poorly differentiated head and neck carcinomas. HPV DNA sequences are determined in 47.6% of analyzed head and neck tumors. The presence of HPV in these tumors suggests their potential etiological role in the development and progression of head and neck carcinomas.
HPV ; head and neck carcinomas ; histological grade ; dot-blot hybridization
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