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Modification of Liver Regeneration and Lymphatic Homeostasis after In Vivo Depletion of CD4+ and/or CD8+ Lymphocytes in Partially Hepatectomized Mice

To establish the role of T-lymphocyte subpopulations in the processes of liver regeneration following partial hepatectomy (pHx)and vice versa, the effect of factors produced in regenerating liver on lymphatic tissue, a series of studies has been conducted.Specifically, in this work we have analyzed (a) the consequences of in vivo depletion of CD4+ and CD8+ lymphocytes on hepatic DNA, RNA, and proteins ; and (b) the consequences of pHx on phenotypic profile of cells in spleen of mice depleted of CD4+ and CD8+ lymphocytes. The data revealed that depletion of CD8 and depletion of both CD4-and CD8-positive T lymphocytes significantly augment the content of hepatic proteins and RNA in regenerating liver. The latter treatment translocated also the peak of protein synthesis from second to firs post-Hx day. On the other hand, pHx stimulated the recovery of CD4+ cells in CD4-depleted mice and augmented the proportion of CD8+ cells in both CD8- and CD4+CD8-depleted mice. The data point to the existence of hepatoregulatory lymphatic cells or cytokines and to the hepatic origin of substances that lead to the induction of lymphocytes with morphogenetic properties.

liver regeneration; lymphatic homeostasis; in vivo depletion; CD4+ lymphocyte; CD8+ lymphocyte; partial hepatectomy

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