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Phylogenetic relationships within the genus Orobanche as revealed by RAPD analysis

RAPD markers were used to study variation among 51 populations belonging to 20 taxa in the genus Orobanche: O. alba, O. amethystea, O. arenaria, O. ballotae, O. cernua, O. clausonis, O. cumana, O. crenata, O. densiflora, O. foetida, O. foetida var. broteri, O. gracilis, O. haenseleri, O. hederae, O. latisquama, O. mutelii, O. nana, O. ramosa, O. rapum-genistae and O. santolinae. A total of 202 amplification products generated with five arbitrary RAPD primers was obtained and species-specific markers were identified. Distance matrices and dendrograms were generated using Dice coefficient and neighbor-joining cluster analysis. The pattern of interspecific variation obtained is in general agreement with previous taxonomic studies based on morphology, and the partition into two different sections (Trionychon and Orobanche) is generally clear. However, the position in the dendrogram of O. clausonis did not fit this classification since it clustered with members of section Trionychon. Within this section, O. arenaria was relatively isolated form the other members of the section: O. mutelii, O. nana and O. ramosa. Within section Orobanche, all O. ramosa populations showed a similar amplification pattern, whereas differences among O. crenata populations growing on different hosts were found. O. foetida and O. densiflora clustered together, supporting the morphological and cytological similarities and the host preferences of these species.

Orobanche; RAPD; phylogenetic relationships; genetic diversity

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