engleski

Lack of p53 and nm23-HI Genes Deletion in Oral Epithelial Keratoses

In our attempt to identify genetic changes involved in epithelial cell proliferation, in certain cases as an earliest step in malignant epithelial neoplasm development, we examined p53 (PCR/RFLP) and nm23-HI (PCR/LOH) allelic deletions in oral benign, potentially malignant epithelial lesions. The study group comprised of 25 benign epithelial lesions, clinically diagnosed as lichen planus (N=17) and leukoplakia (N=8). Two reccurent aphtous ulcers as well as one specimen diagnosed as benign migratory glossitis were included in the study. Among 21 samples analyzed for exon 4 (p53 gene) LOH, only 6 (28, 5%) were informative with no presence of loss of either allele. Of 23 samples tested for LOH at intron 6 of p53 gene, 8 (42, 1%) were informative, again with no specific restriction site. The frequence of alleles was 73, 4% and 26, 6% for alleles with and without a specific restriction site. For nm23-HI gene the analysis was performed on total of 24 cases. Of them 16 (16, 6%) were informative, however, non sample exhibited LOH at his locus. In conclusion, whereas the presence of gross gene alterations (LOH) would have been definitive evidence for the p53 and/or nm23 involvement in hyperproliferation process, the absence of LOH does not exclude the presence of either smaller mutations, or dysregulation of normal gene, or dysfunction at the level of wild type protein. Alternatively, p53 and nm23-HI have no relation to oral lesion formation, and cannot be considered as an early step in benign maybe neoplastic tissue transformation.

p53; nm23-HI; PCR/RFLP; PCR/LOH; oral keratoses

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