engleski

Influence of ochratoxin A treatment on the activity of membrane-bound enzymes in rat brain regions

Ochratoxin A (OA) is a mycotoxin produced by Aspergillus ochraceus and is a natural contaminant of mouldy food. It can be found in fruit and vegetables and in the blood of animals that have been fed with contaminated plant feed in endemic areas of the Balkans, Scandinavia, Germany, France and Canada. We examined the neuroactive potential of OA by measuring the changes in activities of several membrane-bound, cytoplasmic and lysosomal enzymes in the brain of adult female rats, following administration of OA. The activities of both soluble and membrane-bound fractions of 5'-nucleosidase, Ca/Mg ATPase, alanine animopeptidase, gamma-glutamyltransferase, lactate dehydrogenase and of N-acetyl-beta-D-glucosaminidase were followed. Biochemical effects were examined in the cerebral cortex, cerebellum and hippocammpus. The results showed significant alterations in the activity of the enzymes tested. The changes were found to be time dependent and tissue selective. Compared to the controls, a statistically significant increase in gamma-glutamyltransferase was observed in all three brain regions, while in the case of alanine aminopeptidase activities differed with regard to region, the highest increase being observed in the hippocampus. Ca/Mg ATPase and 5'-nucleosidase showed distinct changes lasting for 20 days of treatment, while increases in the activities of N-acetyl-beta-D-glucosaminidase and lactate dehydrogenase were apparent only at the beginning of the treatment. By the end of the trail, the activities of almost all enzymes returned back to normal values.

Ochratoxin a; brain; membrane-bound enzymes; 5-nucleosidase; Ca/Mg ATPase; alanine aminopeptidase; gamma-glutamyl transferase; lactate dehydrogenase

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