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Modelling and optimization of the (R)-(+)-3,4-dihydroxyphenyllactic acid production catalyzed with D-lactate dehydrogenase from Lactobacillus leishmannii using genetic algorithm

A mathematical model for the enzymatic synthesis of (R)-(+)-3, 4-dihydroxyphenyllactic acid (DHPL) was developed. The synthesis was catalyzed by D- lactate dehydrogenase from Lactobacillus leishmannii. Since this enzyme requires NADH as a coenzyme, formate dehydrogenase system was used for NADH regeneration. Optimization of reaction conditions for DHPL synthesis was performed using the genetic algorithm (GA). The optimal initial conditions were found, i.e. the concentration of 3, 4-dihydroxyphenylpyruvate (DHPP), NAD+, formate, and enzyme proportion (D-lactate dehydrogenase and formate dehydrogenase). The genetic algorithm as a flexible optimization tool had been used to obtain the experimental conditions where maximal volumetric productivity could be achieved.

enzymatic synthesis ; mathematical modelling ; optimization ; genetic algorithm

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