engleski

QTL analysis of alpha acid content in hops (Humulus lupulus L.)

An F1 population from the cross between the German variety cv. 'Magnum' showing high levels of alpha acids and a wild Slovene male hop 2/1 was employed to develop genetic maps of the parentals based on AFLP and SSR markers and to identify QTLs associated with alpha acid content. The variability of alpha acid content was evaluated in Žalec, Slovenia in the 2002, 2003, and 2004. The maternal map consisted of 90 markers mapped on 13 linkage groups covering 590 cM of hop genome. The paternal map included 70 markers assigned to 12 linkage groups covering 446 cM. Four putative QTLs (alpha1, alpha2, alpha3, and alpha4) were identified on linkage groups LG03, LG01, LG09, and LG03 of the female map, respectively. Alpha1 was detected in the year 2003 only, whild the rest of the QTLs was identified solely in the year 2004. QTLs explained from 11.9 to 24.8% of the phenotypic variance. The most promising QTL to be used in Marker-Assisted Selection (MAS) process is alpha2 having the 2-LOD confidence interval of only 4 cM with the peak located exactly on the position of the AFLP marker E-ACG+M-CTT/440. The saturation of the map, particularly in the regions of putative QTLs, will be carried out using additional SSR markers. The stability of the QTLs will be tested in next years.

Humulus lupulus L.; alpha acid content; genetic mapping; quantitative trait loci; quality

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