engleski

QTL analysis of broomrape resistance in faba bean (Vicia faba L.)

In order to identify quantitative trait loci (QTLs) controlling broomrape (Orobanche crenata Forsk.) resistance in faba bean (Vicia faba L.) linkage maps were developed using both F2 and RIL (Recombinant inbred lines) populations derived from the cross between a susceptible (Vf6) and resistant line (Vf136). F3 lines, obtained by selfing F2 individual plants, were studied for broomrape resistance under field conditions. RILs were evaluated for broomrape resistance in the field trials carried out in Córdoba in 2003 and 2004 and in Mengíbar in 2004. The linkage map developed in F2 mapping population covered 1445.5 cM of the faba bean genome. More saturated linkage map has been developed in RILs covering 2.811 cM. A high degree of correspondence between maps has been observed. Three putative QTLs controlling broomrape resistance were identified in F2 population. Two out of these were also detected in RILs. Moreover, new genome regions on chromosome I conferring resistance has been detected. Further validation of putative QTLs across multiple test environments will be carried out as well as the saturation of genomic regions associated with broomrape resistance.

Vicia faba L.; Orobanche crenata Forsk.; genetic mapping; quantitative trait loci; resistance breeding

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