engleski

Mapping of QTLs controlling horticultural traits in diploid roses

A segregating population consisting of 96 diploid F1 hybrids between Rosa chinensis var. "Blush Noisette" (D10) and R. wichuraiana (E15) was used to construct a genetic linkage map of the rose genome following 'pseudo-testcross' mapping strategy. A total of 133 markers (130 RAPDs, one morphological and two microsatellite markers) could be located on the 14 linkage groups of the two maps, covering total map lengths of 388 and 260 cM, respectively. Due to the presence of the common, biparental markers the homology of four linkage groups (D10-1/E15-1 to D10-4/E15-4) could be inferred. Three horticulturally interesting quantitative traits (flower size, days to flowering, and leaf size) and the resistance to powdery mildew were analysed in the progeny in order to map quantitative trait loci (QTLs) controlling these traits. A total of 13 putative QTLs have been identified, four controlling flower size, two associated with flowering time, five affecting leaf size, and two conferring resistance to powdary mildew. Possible homologies between QTLs detected using both D10 and E15 maps could be established in case of Fs1/Fs3, Fs2/Fs4, and Ls1/Ls3.

Rosa; molecular markers; genetic map; pseudo-testcross; QTLs

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