engleski

Molecular diversity of hops (Humulus lupulus L.)

Genetic structure and diversity in hop was assessed using 25 new microsatellite markers and a set of 67 wild and cultivated hop accessions. A Bayesian clustering method placed the accessions into five gene pools that correspond to major breeding groups and identified accessions showing mixed origin. A Fitch-Margoliash phylogenetic tree was in agreement with the results of the Bayesian clustering and the available pedigree information. Eight accessions that differed substantially from the rest and were omitted from Bayesian analysis were assigned into two additional gene pools. The seven defined gene pools were best represented by 50 accessions containing in total 246 alleles, of which 120 were 'private alleles' indicating very high diversity in some of the gene pools. The cultivated hops differ significantly from the wild ones, although most of the variability was found within groups. Molecular variances within groups of cultivated and wild hops were homogeneous, suggesting that a similar level of molecular variability is found in both groups of accessions. Core sets of cultivated hop accessions that capture maximum allelic richness were defined, generally including hop cultivars of 'mixed origin' that combine alleles originating from different gene pools and thus have the greatest allelic richness.

Humulus lupulus L.; microsatellites; genetic diversity; genetic structure; core set

nije evidentirano