Isolation and purification of a substance immunologically cross-reactive with insulin (SICRI) from tumor tissue (CROSBI ID 114738)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Levanat, Sonja ; Pavelić, Krešimir
engleski
Isolation and purification of a substance immunologically cross-reactive with insulin (SICRI) from tumor tissue
A substance immunologically cross reactive with insulin (SICRI)was isolated and purified from murine melanoma B16 and myeloid leukemia. Monospecific anti-insulin immunoglobulin C was coupled with CNBr-activated Sepharose 4B to yield an adsorbent. The immunoaffinity column was used to isolate SICRI from tumor tissue. Purified SICRI yielded a single band with mol wt 158 000 on SDS-PAGE. After non-denaturing conditions SICRI appeared again in one single peak. Affinity purified SICRI was shown to be a potent growth factor for different human and murine transformed and normal cells. Biochemical and biological data provide evidence that SICRI and insulin are two distinct biologically active agents.
substance immunologically cross reactive with insulin ; SICRI ; tumor
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Podaci o izdanju
21 (5)
1989.
509-515
objavljeno
0020-711X
10.1016/0020-711X(89)90130-4
Povezanost rada
Temeljne medicinske znanosti