engleski

Immunophenotyping of inflammatory cells in atopic Dermatitis skin lesions

The purpose of the study: Atopic dermatitis (AD) has stimulated many authors to use various diagnostic procedures to obtain new data that would help elucidate the pathogenesis of the disease. The purpose of this study was to perform in cell immunophenotyping and to analyze the inflammatory cell surface markers in the biopsies of skin lesions from 15 AD patients and 5 healthy subjects. Methods. Immunohistochemical analysis was performed in a group of AD patients and compared with that in a control group of healthy subjects. Avidin-biotin immunoperoxidase staining of paraffin embedded 4-mm skin sections, with semiquantitative counting of cells labeled with anti-CD3, anti-CD8, anti-CD20, anti-HLA-DR (HLA, human leukocyte antigen), and anti-immunoglobulin E (anti-IgE) primary antibodies was used. Summary. The results of AD skin analysis showed a greater infiltration of CD3+ lymphocytes, especially of CD4+ subtype, compared with CD8+ lymphocytes. AD skin biopsy specimens also showed a higher intraepidermal HLA-DR+ Langerhans' cell count, the presence of HLA-DR on lymphocytes in the dermis, and higher intraepidermal expression of IgE+ cells compared with healthy controls. Conclusions A statistically significant difference (P<0.05) was found between the two groups for intradermal and intraepidermal CD3, CD4 and HLA-DR, intradermal CD8, and intraepidermal IgE+ cells. Immunophenotyping was found to be a useful diagnostic method in AD patients.

Immunophenotyping; inflammatory cells; Atopic dermatitis

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