engleski

Biological monitoring of genotoxic hazards in workers of the cigarette manufacturing industry

Biological monitoring of genotoxic hazard was performed in 45 workers manufacturing cigarettes and 45 control subjects. The exposed workers were additionally exposed to a source of strontium 90, a strong beta-ray generator that measures or gauges the density of the moving tobacco-containing rod. The control subjects were not exposed to ionizing radiation or chemicals at their working places. Peripheral blood lymphocytes were analyzed for cytogenetic parameters (chromosomal aberrations and the micronuclei frequency). An analysis of variance (ANOVA) was performed to model the relationship between the confounding factors such as sex, age, years of exposure, smoking habits and X-ray diagnostics and chromosomal aberrations and micronuclei frequency. The exposed subjects significantly differed fom controls in double chromosome breaks, acentrics and chromosomal exchanges (tri-radials and tetra-radials), as well as in the total micronucleus count. Total micronucleus count was significantly higher in the group exposed from 21 to 30 years of exposure than in the control group. In the expoed women smokers, only the total micronucleus count was significantly different from controls. The frequency of chromatid breaks was significantly higher in women. The detected increase in cytogenetic endpoints in the exposed workers might be mediated by a possible additive or synergistic action of different chemical agents used in cigarette manufacturing, and not by the genotoxic effect of a single cigarette component.

biomonitoring ; chromosomal aberrations ; cytochalasin B-blocked micronucleus test

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