engleski

In vitro cytotoxicity of root canal sealers

To study cytotoxicity of the root canal sealers Diaket (3M ESPE, Germany), IRM (Dentsply, USA), SuperEBA (Bosworth Company, USA), Hermetic (Pharma Gmbh + Co.KG, Germany) and GuttaFlow (Roeko, Coltene/Whaledent GmbH + Co.KG, Germany) on human larringeal carcinoma cells (HEP) in vitro. The sealers were prepared according to the manufacturer's instructions under aseptic conditions and a 0.02 mL increment of each sealer was placed at the bottom of a 24-well plate. The sealers were covered with a 1x104 cell suspension for 1h, 24h, 48h, 7 days and 1 month after mixing. Four samples and respective controls without sealer were prepared. After 5 days of incubation the number of cells was determined using an electronic counter ; the number of viable cells was determined under light microscopy following the addition of methylen blue. The number of cells in the experimental dishes was calculated as a percentage of the controls. GuttaFlow showed increased cytotoxicity over time after mixing. After one month, cytotoxicity was significantly higher (22%) than after 1h (80%), 24h (68%), 48h (66%) and 7 days (50%)(p<0.01). Other tested materials showed strong cytotoxic effect. There were no viabile cells for all tested periods.All tested materials except GuttaFlow showed strong cytotoxic effect in all tested periods but cytotoxicity for GuttaFlow is dependent on time.

cytotoxicity; sealers

nije evidentirano