engleski

Leakage of bovine serum albumin in root canals filled with IRM and SuperEBA, with or without orthograde filling: an in vitro study using spectrophotometry

Aim was to determine quantitatively the leakage of two apical root-end filling materials: SuperEBA (Staident International, UK) and IRM (DeTrey, Dentsply, Germany) in root canal samples with or without orthograde filling, by evaluating Bovine Serum Albumin (BSA) microleakage using spectrophotometric method. Thirty five single-rooted teeth were instrumented and divided into five groups. The apices of all teeth were resected. Root-end cavities in the samples from the first two groups, without orthograde root canal filling, were filled with SuperEBA (Group 1) and IRM (Group 2). The samples from the Groups 3, 4 and 5 were filled conventionally with gutta-percha and sealer, and after setting for 24 hours, the apices were resected. The teeth from Group 3 did not receive a root-end cavity preparation and filling. In the samples from Groups 4 and 5 root-end cavities were prepared and filled with SuperEBA (Group 4) and IRM (Group 5). Each sample was mounted in the glass vial and exposed to the BSA protein solution. Two sets of spectrophotometric measurements were conducted, after 7 and 60 days. After 7 days microleakage was observed in only two specimens. After 60 days microleakage was recorded for all specimens. The greatest mickroleakage was observed in Group 2 (0.0041± ; ; 0.0000011 µ ; ; g), then in Group 3 (0.0034± ; ; 0.0000064 µ ; ; g) and Group 1 (0.0026± ; ; 0.0000019 µ ; ; g). Samples from group 4 and group 5 leaked the least and to the same extent (0.0007± ; ; 0.0000014 µ ; ; g). ANOVA analysis showed that there was a significant difference between the groups (F=7.054 ; p=0.0000428) and the Tuckeys analysis showed statistically significant difference (p<0.05) between Groups 2 and 4, Groups 2 and 5, Groups 3 and 4, and Groups 3 and 5. Significantly less leakage occurred in samples filled with orthograde and root-end fillings than in the samples filled only with a orthograde approach and the samples with IRM root-end fillings.

SuperEBA ; IRM ; spectrophotometry ; BSA

nije evidentirano