Production of L-malic acid by permeabilized cells of commercial Saccharomyces sp. strains (CROSBI ID 115864)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Vrsalović Presečki, Ana ; Vasić-Rački, Đurđa
engleski
Production of L-malic acid by permeabilized cells of commercial Saccharomyces sp. strains
Of various yeasts tested in the conversion of fumaric to L-malic acid, Saccharomyces bayanus had the highest activity of fumarase. Cells permeabilized with 0.2 %(w/v) CTAB for 5 min gave maximum enzyme activity. Under non-growth conditions, fumarase activity in the permeabilized cells was four times higher (271 U/g) than that of the intact cells (67 U/g). The proposed mathematical model for the batch production of L-malic acid was validated at different initial fumaric acid concentrations. The average conversion of fumaric acid was up to 82 % and gave 21, 40, 83 and 175 mM L-malic acid from respectively, 25, 50, 100 and 210 mM fumaric acid.
fumarase ; L-malic acid ; modelling ; permeabilization ; yeast
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Podaci o izdanju
27 (23-24)
2005.
1835-1839
objavljeno
0141-5492
1573-6776
10.1007/s10529-005-3890-3
Povezanost rada
Biotehnologija, Kemijsko inženjerstvo