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Antifungal activity of fluid extract and essential oil from anise fruits (Pimpinella anisum L., Apiaceae)

Antifungal activities of fluid extract and essential oil obtained from anise fruits Pimpinella anisum L. (Apiaceae) were tested in vitro on clinical isolates of seven species of yeasts and four species of dermatophytes. Diffusion method with cylinders and broth dilution method were used for testing of antifungal activity. Anise fluid extract showed antimycotic activity against Candida albicans, C. parapsilosis, C. tropicalis, C. pseudotropicalis and C. krusei with MIC values between 17 and 20 % (V/V). No activity was noticed against C. glabrata, and anis fruits extracts showed growth promotion activity on Geotrichum spp. Anise fruits extract inhibited growth of dermatophyte species (Trichophyton rubrum, T. mentagrophytes, Microsporum canis and M. gypseum) with MIC's values between 1.5 and 9% (V/V). Anise essential oil showed strong antifungal activity against yeasts with MIC's lower than 1.562 % (V/V) and dermatophytes lower than 0.781 % (V/V). There are significant differences in the antifungal activities between anise fluid extract and anise essential oil (p<0.01). Anise essential oil exhibited stronger antifungal activities against yeasts and dermatophytes with MIC values between 0.097 and 1.562 % (V/V), respectively.

Anise; anise fruits; Pimpinella anisum; Apiaceae; fluid extract; essential oil; antifungal; Candida; dermatophytes

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