engleski

Production and Isolation of Keratinase from Filamentous Fungi

Keratinase is a proteolytic enzyme which catalyses the degradation of the keratin. Most of the purified kertinase preparations hydrolyse a wide range of proteins, such as bovine serum albumin, collagen, elastin, hemoglobin, myoglobin, casein etc. They have higher proteolytic activity than most of other proteinases. The biosynthesis of the keratinase in the microorganism is reported to be induced by the presence of keratin, as the only source of carbon and nitrogen in the medium. Fermentations of filamentous fungi were performed in the shaking flasks, for a few days, at the temperature of 30C. The soybean-flour was used as an inducer. The filtration and the centrifugation were used for the isolation of the enzyme from the fermentation broth. The supernatant was concentrated by ultrafiltration and the retentate was lyophilized. The obtained crude keratinase was tested for the keratin degradation activity using statum corneum from human sole as the enzyme substrate. By mesaurments of the soluble protein content the specific activity of the crude fungal keratinase could be detremined.

keratinase

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