engleski

Cytotoxicity of nanosize V2O5 particles to selected fibroblast and tumor cells

Two kinds of nanosize V2O5 particles were synthesized in our own laboratory and concomitantly applied to V79 and L929 fibroblasts and SCCVII, B16F10 and FsaR tumor cells. The morphologies of the cells were monitored using an inverted inverse microscope equipped with digital camera, while quantitative determination of the cytotoxicity of nanosize V2O5 particles was measured using crystal violet bioassay. 24 hours after the addition of nanosize V2O5 particles (20  M), noticeable changes in the morphology and density of fibroblast and cancer cells were observed. Reculturing in a freshly prepared medium for the next 24 hours showed a high recovery effect on V79, SCCVII and B16F10 cells, while FsaR and L929 cells were seriously damaged and unable to recover. At a higher concentration of nanosize V2O5 particles (100  M), the cytotoxicity of V2O5 prevailed against the recovery effect in all cell types. Quantitative measurements have shown that the resistance of investigated cell cultures to the cytotoxicity of nanosize V2O5 particles decreases in the order V79 > SCCVII > B16F10 > FsaR > L929. The high cytotoxic effect found on FsaR cells suggests that nanosize V2O5 particles could be regarded as poisoning material in the treatment of FsaR fibrosarcoma cells. Possible mechanisms involved in the cytotoxicity of nanosize V2O5 particles were discussed.

V2O5 ; citotoxicity ; nanoparticles ; glucose ; fibroblasts ; tumor cells ; V79 ; SCCVII ; B16F10 ; FsaR ; L929.

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