Application of the alkaline comet assay in biodosimetry:Assessment of in vivo DNA damage in human peripheral leukocytes after a gamma radiation incident (CROSBI ID 116179)
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Podaci o odgovornosti
Garaj-Vrhovac, Vera ; Kopjar, Nevenka ; Ražem, Dušan ; Vekić, Branko ; Miljanić, Saveta ; Ranogajec-Komor, Maria
engleski
Application of the alkaline comet assay in biodosimetry:Assessment of in vivo DNA damage in human peripheral leukocytes after a gamma radiation incident
The alkaline comet assay was employed in the assessment of DNA damage in leukocytes of a worker incidently exposed to gamma radiation (221mSv, Co-60 source). The comet tail lenghts and tail moments were studied. By using the alkaline comet assay immediately after accidental exposure a high level of DNA damage was recordd one day and one week after the radiation incident. Later on, a decrease in both comet parameters was diminished during a one year period, it was still elevated compared to normal values recorded in leukocytes of a healthy, unexposed person.The results, obtained indicate that the alkaline comet assay is a rapid and sensitive microdosimetric technique and is suitable for in vivo human biomonitoring, especially in cases of incidental exposure to ionizing radiation.
cell gel-electrophoresis; white blod cells; ionizing radiation; human lymphocytes; individual cells; base damage; exposure; induction; cancer; repair
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