Alkaline comet assayin human biomonitoring of occupational exposure to physical and chemical mutagens (CROSBI ID 116195)
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Podaci o odgovornosti
Garaj-Vrhovac, Vera ; Kopjar, Nevenka ; Želježić, Davor
engleski
Alkaline comet assayin human biomonitoring of occupational exposure to physical and chemical mutagens
The alkaline comet assay was selected as a biomarker of exposure to evaluate the ongoing occupational exposure to physical and chemical mutagens. The primary DNA damage was evaluated by measuring the extent of DNA migration in peripheral blood leukoctes. Parameters of comet assay were studied in 50 medical workers occupationally exposed to ionizing radiation, 10 radar facility workers exposed to microwave radoation, 50 medical workers handling antineoplastic drugs, 10 workers employed in pesticide production, and in 50 corresponding unexposed control subjects. It was found that the subjects who were occupationally exposed to physical and chemical mutagens for various periods of time showed highly significant increase in levels of DNA damage compared to the controls. In some occupationally exposed subjects smoking habit significantly elevated the level of primary DNA damage. The results obtained have confirmed the usefulnedd of the alkaline comet assay as an additional complement to the standard biodosimetric method, reflecting the current level of DNA damage and/or repair in peripheral blood lymphocytes.
alkaline comet assay; leukocytes; biomonitoring; occupational exposure; ionizing radiation; microwave radiation; antineoplastic drugs; pesticides
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Podaci o izdanju
18 (1)
2003.
265-271-x
objavljeno
1219-1221