engleski

In vitro and in planta Agrobacterium tumefaciens madiated transformation of Arabidopsis thaliana

Cell transformation of Arabidopsis thaliana (L.) Heynh. with Agrobacterium tumefaciens (LBA4404)was performed in two ways: in vitro - by the transformation of root and stem explants - and in planta - by the transformation of whole plants in the greenhouse. Agrobaacterium contained the binary plasmid pBI121 or its constructs with the fragments of carrot DC-8 promoter. The results show that transformation efficiency in vitro is influenced by the age and type of explant. It was not possible to induce transgenic calli on eexplants of 5 week old Arabidopsis roots, although it was possible on explants of 3 week old roots. Although green calli grew on Arabidopsis stem explants, transgenic shoots did not develop. When using traansformation in vitro, from seed to mature primary transformant it took 15 to 16 weeks and for in planta transformation it took 19 to 20 weeks.

Cell transformation; Arabidopsis thaliana; in vitro; in planta; Agrobacterium tumefaciens LBA4404

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