engleski

Nm23-H1 and colon adenocarcinoma

Nm32 gene was originally identified by differential hybridisation of K-1735 melanoma cell line clones of varying metastatic potential. A tumor metastases-supressor function was implicated by the reduced expression of nm23 in highly metastatic sublines compared with non-metastatic sublines derived from the same K-1735 clone. The purpose of this study was to evaluate whether the expression of the nm23-H1 protein or loss of heterozygosity (LOH) of the nm23-H1 gene is associated with tumor stage and grade of tumor differentiation. We also investigated the correlation of nm23-H1 expression with 5-year survival. Of 102 adenocarcinomas that were examined, 41% showed a weak positive immunostaining for nm23-H1 protein. The most nm23-H1 positive tumors were in Dukes' B (67%) and in the well differentiated tumors (65%). Statistical analysis showed that there was no statistically significant difference in survival between the patients with nm23-H1 positive tumors and patients with tumors that were not stained for nm23-H1 protein. To analyze LOH at the nm23-H1 gene we used VNTR marker located in untranslated 5' region of the nm23-H1. At this nm23-H1 locus 60% of samples were informative and 33% of them demonstrated LOH. The nm23-H1 was more frequent in tumors that were larger than 5 cm than in smaller ones. Positive correlation was found between the nm23-H1 LOH and histological grade as well as between the nm23-H1 LOH and Dukes' stage of tumor samples.

nm23-H1; colon adenocarcinoma

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