engleski

Distribution of immune cells expressing CD3a, CD21 and S-100 protein markers in the porcine gut-associated lymphoid tissues

In swine, the distribution of immune cells within the gut-associated lymphoid tissues (GALT) is highly organized. The appearance of such cells could not be separated from the effects of age, weaning and exposure to environment. Here, we have examined the distribution patterns of a subset of CD3a^+ T and CD21^+ B cells as well as S-100 protein^+ cells and secretory (s) IgA^+ cells within GALT compartments (such as jejunal lamina propria = JLP, ileal Peyer’s patches = IPP, and mesenteric lymph node = MLN) of juvenile 8-week-old conventionally reared pigs using two either monoclonal antibodies (mAbs) or polyclonal antibodies (pAbs) in the immunohistochemical staining techniques with avidin-biotin complex (ABC) or peroxidase-antiperoxidase complex (PAP), respectively. The most potent porcine T-cell marker - CD3 surface antigen - is expressed as CD3a epitope on ileal intraepithelial lymphocytes, and numerous lymphocytes in the extrafollicular areas of MLN and dome region of IPP. Conversely, the cells expressing CD21 surface molecule were only demonstrable in the interfollicular areas of MLN and in the germinal centers of IPP. A strong reaction to sIgA was displayed by the plasma cells in the lumen of crypts and those residing the lamina propria of jejunum and ileum. The S-100 protein^+ cells were numerous in JLP around the crypts and in IPP of weaned pigs. Both applied mAbs showed to be useful reagents for phenotypic and functional analyses of porcine lymphoid cell subsets by the ABC technique. However, further investigation of S-100 protein marker is needed to determine which (if any) subset of porcine CD3^+ CD4^- CD8^+ T cells could be designated as orthologue of human CD8^+ CD11b^+ suppressor T cells.

Gut lymphoid tissues; CD3a; CD21; S-100 protein and IgA positive cells; pigs

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