S-100 protein marker coexpressed on porcine gut immune cells is not orthologue of human CD8^+ CD11b^+ S-100^+ suppressor T lymphocytes (CROSBI ID 468400)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Lacković, Gordana ; Cvetojević, Maja ; Valpotić, Ivica
engleski
S-100 protein marker coexpressed on porcine gut immune cells is not orthologue of human CD8^+ CD11b^+ S-100^+ suppressor T lymphocytes
We have demonstrated the expression of CD3a and S-100 protein molecules on porcine immune cells populating the gut-associated lymphoid tissues (GALT). The distribution patterns of CD3a^+ and S-100 protein^+ cells within GALT compartments, such as jejunal and ileal lamina propria (JLP and ILP), and ileal Peyers patches (IPP) of juvenile 8-week-old, conventionally reared pigs were examined using double staining immunohisichemical methods. The samples of jejunum and ileum were fixed in methanol-chloroform-acetic acid and embedded in paraffin and processed for immunohistochemical staining. The S-100 protein marker was detected by polyclonal antibodies (pAbs) using peroxidase-antiperoxidase (PAP) staining, and subsequently, the CD3a antigen was identified by monoclonal antibodies (mAbs) using avidin-biotin complex (ABC). The use of rabbit anti-bovine S-100 protein pAbs, in the PAP method, revealed numerous mononuclear cells around the crypts of JLP and ILP, and in the IPP. Solitary S-100 protein^+ cells were present among capillary endothelial cells of JLP and ILP. In the ABC method, using mAbs against CD3a epitope of porcine T cells, we have found that the most numerous CD3a^+ cells were intraepithelial lymphocytes and T cells between IPP. These cells were rarely distributed in the lamina propria and practically absent in the crypts. Double positive, CD3a^+ S-100 protein^+ T cells were sporadically (if any) demonstrable in the ILP, and thus, their assignment as porcine orthologue of the human CD8^+ CD11b^+ S-100 protein^+ suppressor lymphocytes seems to be unlikely. Hence, their role in down regulation of porcine intestinal immune responses still remains to be definitely established.
Gut-asociated lymphiod tissue; porcine; S-100 protein; immunochistochemical staining; suppressor T lymphocytes.
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Podaci o prilogu
79-79-x.
1998.
objavljeno
Podaci o matičnoj publikaciji
Vitale, Branko
Zagreb: Hrvatsko prirodoslovno društvo
Podaci o skupu
The 4th International Meeting "Mechanisms in local immunity"
poster
16.09.1998-19.09.1998
Opatija, Hrvatska