engleski

Characterization of two brown haphthalane preparations

Today, there are several variations of the naphthalane preparations used as healing agents. The most improved version among them, there is a transparent, colorless, slightly pine-like odor type. Being non-aromatic and at the same time being relatively very high in steranes that are thought to be responsible for curing effect, this naphthalane preparation is marked as NAVS (Non-Aromatic, Very Sterane high) Naphthalane. It was proved to be highly bioactive and to meet all pharmacopoeias requirements regarding aromatics content (absence). Even though, for relatively lower price, brown naphthalanes containing aromatics, are still in ordinary use. Thousands of patients, who have been passing through the brown naphthalane therapy, deserve thorough and permanent study of brown naphthalane composition. For that reason, two the most frequently used brown naphthalane preparations, one of the Azerbaijani (N1) and one of the Croatian (N2) origin, were studied. The N1 and N2 samples underwent the bulk properties and some chemical composition analyses as well as some focused constituent identification. For the composition complexity, prior to the compound identification, N1 and N2 were fractionated into fractions of saturated aliphatics (SATf), of aromatic compounds (AROf) and of the compounds containing polar functional groups (POLf), using mobile phase of increasing polarity, applied on open air preparative liquid chromatography column (LC). In AROfs there was the group type composition determined by a normal phase high performance liquid chromatography (NP HPLC). Gas chromatography-mass spectrometry (GC-MS) was applied for SATfs and AROfs, in order to analyze some focused constituents. Published mass spectra and mass fragmentogram fingerprints served in identification. Both of the samples were found to be unresolved complex mixtures (UCM), relatively poor in n-alkanes. A large number of isoalkanes, cycloalkanes as well as partly/fully aromatic oligocyclics incl. fused aromatic rings, in a great variety of isomers and homologs made the majority of both of the samples. N1 was a dark, viscous, non-transparent fluid with a strong petroleum smell. Aromatics, which made 54% mas. of it, were found in the range of mono-, di- and tri+. N1 had well presented steroids, including steranes, contained a whole range of hopanes and lower oligocyclics. N2 was found to be pale brown liquid, with smell similar to gasoline. It contained somewhat lower percentage of aromatics (50% mass.) ; also relatively lower percentage of tri+ fused aromatics. Relatively it constituted lower content of steroids (especially higher ones) contained almost no hopanes, while different oligocyclics were well presented. It is important to learn the chemical composition of the brown naphthalane preparations since it influences their healing potential (steranes), and invites for precaution (fused aromatic compounds

Brown naphthalane; Azerbaijani naphthalane; Croatian naphthalane; naphthalane characterization

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