engleski

Sensitivity of Indirect Immunofluorescednce Test in the Diagnosis of Pemphigus

Indirect immunofluorescednce testinf of sera from patients with pemphigus produces a postivie intercellular staining on a variety of epithelial substrates with different sensitivity. We aimed to determine the sensitivity of indirect immunofluorescence (IIF) test in detecting pemphiogus antibodies, using two different substrates: guinea pig lip and human skin. IIF detected antibodies in 66 out of 109 patients with different types od pemphigus. Sensitivity of IIF performed with guinea pig lip was 40%, while with human skin it increases to 69%. However, we found that neither human skin nor guinea pig lip was sensitive enough to make an IIF test reliable for the diagnosis of pemphigus.

(Indirect immunofluorescence; pemphigus; substrate)

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