engleski

Ethanol induced changes in HDL oxidation studied by nonradiative energy transfer fluorescence

The aim of the study was to follow the in ƀuence of alcohol on the structure and function of serum high density lipoproteins, HDL. The effect of different concentrations of ethanol on the HDL oxidation process was monitored by ƀuorescence spectroscopy of apoA-1 tryptophans and molecular probe 6-propionyl-2-dimethil-aminonaphtalen, PRODAN. PRODAN, although predominately lipophilic, participates in environments of different polarity, in HDL monolayer and in water media. The mathematical model for calculating the spectra of PRODAN only in lipid matrices of HDL was developed. The changes provoked at lipid/protein interface, at different stages of particle oxidation, were studied by non-radiative energy transfer, NRET. The concentration of ethanol and the stage of HDL oxidation modulate the process of energy transfer. The increase of ethanol concentration disrupts the communication over the boundary between protein and lipid monolayer as seen by NRET. The process of oxidation is slowed down with the increase of ethanol concentration. In fully oxidative state HDL is more sensitive to the ethanol induced perturbation.

HDL; ethanol; fluorescence

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