engleski

Functional analysis of Stam2 in mouse using gene trap method

Stam2 (Signal transducing adaptor molecule 2) was identified as a phosphotyrosine protein involved in cellular response to variety of cytokines and growth factors. Together with Hrs (hepatocyte growth factor regulated tyrosine kinase substrate) and Eps15 (EGFR pathway substrate clone no. 15) it has been implicated in endosome mediated intracellular membrane trafficking. In order to investigate expression and function of Stam2, mouse carrying a gene trap modification of Stam2 gene was created. Gene trap method involved genetical modification of embryonic stem (ES) cells with a nonhomologous DNA vector containing a splice acceptor and fused promotorless lacZ and neoR genes. After a large scale screen of obtained ES cells, corresponding mouse lines were produced, and the line carrying modification of Stam2 gene was selected for further investigation. Stam2 expression during development was detected after E9.5 in the gut, notochord, neural tube and heart. In the nervous system it was located in the floor, roof and basal plates of the developing neural tube, and in the cortex, hippocampus and olfactory bulb of the telencephalon. Toward the end of gestation, Stam2 expression appeared in the testis and ovary, pituitary and adrenal glands, skin and epithelium of oral cavity and tongue. As the expression of Stam2 indicated its possible function in the nervous system, general health and behavior of Stam2gt homozygotes was compared to wild type C57Bl/6 mice. Homozygous mutant males had lower body mass, and both sexes preformed poorly in olfactory test. This indicates possible Stam2 function in regulation of body mass and sense of smell.

Stam2; gene trap; mouse; embryo

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